Array of fluorophores using a silica gel-based, high-throughput combinatorial platform. Part I: instrumental device and measurement
The environment surroundings of a fluorophore affect its fluorescence due to non-specific (polarity) interactions. Several mathematical models have been proposed to relate the Stokes´ shift with the dielectric constant (e), the index of refraction (n) and/or other thermodynamic parameters characterizing the polarity of a medium. However, very little attention has been devoted to the environment effect in the fluorescence quantum yield (i.e. fluorescence intensity), but this effect applies for a lot of compounds interacting with many fluorophores. This has been used as the basis of detection systems for liquid chromatography and chemical sensors.
As a new analytical application, an array of 49 fluorophores (prepared on a silica gel plate) is here developed, aiming to display their interactions with potential analytes in a high throughput format to get a specific pattern response (fingerprint). Cholesterol and sphingomyelin have been chosen as analytes. In this communication (Part I) the experimental device and the very first experimental results are presented.