Absence of SARS-CoV-2 Antibodies in Natural Environment Exposure in Sheep in Close Contact with Humans

Absence of SARS-CoV-2 Antibodies in Natural Environment Exposure in Sheep in Close Contact with Humans. Sergio Villanueva-Saz*, Jacobo Giner, Antonio Fernández, Delia Lacasta, Aurora Ortín, Juan José Ramos, Luis Miguel Ferrer, Marta Ruiz de Arcaute, Ana Pilar Tobajas, María Dolores Pérez, Maite Verde, Diana Marteles, Ramón Hurtado-Guerrero, Julián Pardo, Llipsy Santiago, Andrés Manuel González-Ramírez, Javier Macías-León, Ana García-García, Víctor Taleb, Erandi Lira-Navarrete, José Ramón Paño-Pardo, Héctor Ruíz*. Animals, 2021. DOI: 10.3390/ani11071984

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the zoonotic causative agent of the coronavirus disease 2019 (COVID-19) that it has caused a pandemic situation with million of infected humans worldwide. Among domestic animals, transmissibility and exposure to the infection have been limited studied in natural conditions. Some animals are exposed and/or susceptible to SARS-CoV-2 infection, such as cats, ferrets and dogs. By contrast, there is no information about the susceptibility of ruminants to SARS-CoV-2. This study tested the antibody response in 90 ovine pre-pandemic serum samples and 336 sheep serum samples from the pandemic period (June 2020 to March 2021). In both cases, the animals were in close contact with veterinary student’s community composed of more than 700 members. None of the serum samples analyzed was seroreactive based on enzyme-linked immunosorbent assay (ELISA) using the receptor-binding domain (RBD) of Spike antigen. In this sense, no statistical difference was observed compared to the pre-pandemic sheep. Our results suggest that it seems unlikely that sheep could play a relevant role in the epidemiology of SARS-CoV-2 infection. This study reports for the first time the absence of evidence of sheep exposure to SARS-CoV-2 in natural conditions.

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